섬유

a fiber-optic enzyme sensor for the determination of adenosine diphosphate using internal analyte recycling

  • 출판일1999.03
  • 저자
  • 서지사항
  • 등록일 2016.11.02
  • 조회수 410
an optical multienzyme biosensor for the sensitive determination of adenosine diphosphate (adp) is presented hexokinase, pyruvate kinase, and glucose-6-phosphate dehydrogenase are coimmobilized in a polyurethane membrane and fixed to a commercial fiber-optic probe. adp is continuously phosphorylated/dephosphorylated in a cycle catalyzed by the kinases the glucose-6-phosphate formed in the hexokinase reaction is oxidized by the dehydrogenase, leading to the formation of nadh, the fluorescence of which is detected by the optical transducer and used as the measuring signal. comparison of the responses of the sensor to adenosine triphosphate (atp) and adp shows an amplification factor of 90 for adp measurement. the detection limit is thus lowered to 0.1 mu m, the useful measuring range extending up to 20 mu m. the response of the sensor to other compounds involved in the reaction system has also been investigated
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