섬유

performance of a 2-haloalkanoate dehalogenase immobilized in a hollow fiber reactor

  • 출판일1999.03
  • 저자
  • 서지사항
  • 등록일 2016.11.02
  • 조회수 315
a hollow fiber reactor for the specific dehalogenation of 2-haloalkanoic acids has been developed. cell-free extracts of pseudomonas cepacia mba4 containing dehalogenase iva were immobilized on hollow fibers contained in a glass reactor, and the reactor's efficiency was assessed in comparison with free enzyme activity. at all protein loadings examined, the reactor was under diffusional control. while the v//m//a//x of the enzyme was decreased by immobilization, the sensitivity of the observed reaction rates to temperature also decreased. these findings suggest that this immobilization procedure is an effective method for entrapping the biocatalyst for processes such as the resolution of racemic mixtures of 2-haloalkanoic acids. (author abstract) 19 refs
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