섬유

enhancement in resolution and lack of radiation damage in a rapidly frozen iysozyme crystal subjected to high-intensity synchrotron radiation

  • 출판일1999.03
  • 저자
  • 서지사항
  • 등록일 2016.11.02
  • 조회수 288
x-ray data have been collected from a monoclinic hen egg-white lysozyme (hewl) crystal, rapidly frozen at 120 k on the end of a glass fiber, using high-intensity synchrotron x-radiation. a comparison of oscillation photographs taken at the beginning and end of data collection shows no evidence of radiation damage even though the crystal was exposed in the synchrotron beam for approximately 8 h. not only are the low-resolution data unaffected by radiation damage but the high-resolution data remain unaffected as well. there is also a marked improvement in the resolution of the diffraction pattern of the rapidly frozen crystal when compared to a similarly sized crystal irradiated in a capillary tube at room temperature (author abstract) 9 refs
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