섬유

avidin-biotin coupling as a general method for preparing enzyme-based fiber-optic sensors

  • 출판일1999.03
  • 저자
  • 서지사항
  • 등록일 2016.11.02
  • 조회수 312
a general method for preparing enzyme-based fiber-optic sensors is described. three different sensors, capable of detecting penicillin, organic esters, and urea, were developed based on this method. each fiber was constructed first with a layer of polymer containing pendant amino groups that react further with succinimidyl-derivatized biotin. later biotin and fluorescein-labeled enzymes were introduced to the fiber through a sandwich biotin-avidin-biotin interaction. the substrate concentration is detected indirectly through the ph-sensitive dye, fluorescein, which responds to the micro-environmental ph changes produced by the enzyme catalyzed reactions. the response times for the penicillin, organic ester, and urea optrodes are less than 90, 30, and 70 s, respectively. each sensor has a detection limit of 0.1 mm and responds to substrate concentrations over 2 orders of magnitude (author abstract) 20 refs
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