섬유

fibre-optic sensor with co-immobilised bacterial bioluminescence enzymes

  • 출판일1999.03
  • 저자
  • 서지사항
  • 등록일 2016.11.02
  • 조회수 293
a fiber-optic bioluminescent for the microdetermination of nadh is described. measurements can be performed in the linear range 1 $multiplied by$ 10** $minus$ **9 m $minus$ 3 $multiplied by$ 10** $minus$ **6 m with a detection limit of 3 $multiplied by$ 10** $minus$ **1**0 m using the bacterial luciferase and nad(p)h:fmn oxidoreductase co-immobilized on a preactivated polyamide membrane the relative standard deviation was 4.8-5.5 at 4 $multiplied by$ 10** $minus$ **8m nadh and the steady-state response time was 2 min when stored at $minus$ 20 $degree$ c glycerol, the activity of the bioactive membranes was higher than that measured prior to freezing and then remained stable for more than four months. (author abstract) 29 refs