섬유

preparation and properties of covalently immobilized alkaline phosphatase on bombyx mori silk fibroin fiber

  • 출판일1999.03
  • 저자
  • 서지사항
  • 등록일 2016.11.02
  • 조회수 303
alkaline phosphatase, alpase (e.c.3.1.3.1) was immobilized on bombyx mori silk fibroin fiber by covalent bond formation. two different immobilization procedures (i.e. diazo and cyanogen bromide, cnbr, methods) were tried. the immobilization conditions such as ph, enzyme concentration, reaction time, and temperature were examined in detail, and optimum conditions were obtained. the immobilized enzyme activity was characterized with michaelis constant, k//m, and maximum activity, v//m. the optimum ph of the activity of the fixed enzyme tended to shift to the acid side. thermal stabilities of the enzyme were improved above 50 $degree$ c. in addition, the immobilized enzyme maintains activity over a long period. (author abstract) 22 refs
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