섬유

properties of invertase immobilized on the poly(ethylene-co-vinyl alcohol) hollow fiber membrane

  • 출판일1999.03
  • 저자
  • 서지사항
  • 등록일 2016.11.02
  • 조회수 304
invertase was ionically immobilized on the poly(ethylene-co-vinyl alcohol) hollow fiber inside surface, which was aminoacetalized with 2-dimethylaminoacetaldehyde dimethyl acetal immobilization and enzyme reaction were carried out by letting the respective solutions pass or circulate through the inside of the hollow fiber, and the activity of invertase was determined by the amount of glucose produced enzymatically from sucrose immobilization conditions were examined with respect to the enzyme concentration and to the time, and consequently the preferable conditions at room temperature were found to be 5 $mu$ g/ml of enzyme concentration and 4 h of immobilization time. under those conditions the immobilization yield and the ratio of the activity of the immobilized invertased to that of the native one were 89 and 80 percent respectively. (edited author abstract). 16 refs
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