섬유

immobilized enzymes in blood plasma exchangers via radiation grafting

  • 출판일1999.03
  • 저자
  • 서지사항
  • 등록일 2016.11.02
  • 조회수 265
the enzyme asparaginase was immobilized onto a porous hollow polypropylene (pp) fiber blood plasma exchange device for the treatment of acute lymphocytic leukemia. the devices were first radiation grafted with polymethacrylic acid (poly(maac)). this introduces carboxy groups onto the surface of the fibers. several variables were studied in the grafting reaction including the effects of solvent type and monomer conentration. the carboxy groups were activated with n-hydroxy succinimide (nhs) using carbodiimide chemistry. asparaginase was then covalently immobilized on the activated surfaces. quantitative relationships were found relating the percent graft to the amount of immobilized enzyme which was active. the enzyme reactor was tested both in vitro and in vivo using a sheep as an animal model. (author abstract) 18 refs
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