섬유

theoretical study of continuous nad recycling by conjugated enzymes immobilized in ultrafiltration hollow fiber

  • 출판일1999.03
  • 저자
  • 서지사항
  • 등록일 2016.11.02
  • 조회수 266
the stedy-state solution of continuos conjugated enzyme rections was theoretically obtained by application of the theorell-chance reaction mechanism for the system of alcohol dehydrogenase and lactate dehydrogenase immobilized in an ultrafiltration hollow-fiber tube. the reactor was a dialyzer of complete mixing type. the coenzyme, nad, was dynamically recyled between its oxidized and reduced state by the action of the conjugated enzymes under continuous operation. the results of numerical calculations elucidated the dependence of nad recycle number on such conditions as concentration of immobilized enzyme, feed concentrations of nad and substrates, permeability, reactor volume, flow rate and fractional volume of fiber packed in a reactor with a sufficiently high enzyme concentration and a low nad concentration, a very high nad recycle number ( $greater than$ 1000) with a good conversion of substrate ( $greater than$ 50) was theoretically possible through the strong affinity between the enzymes and the cofactor. 15 refs