섬유

immobilization of ureolytic cells through flocculation and adhesion on cotton cloth using polyethyleneimine

  • 출판일1999.03
  • 저자
  • 서지사항
  • 등록일 2016.11.02
  • 조회수 280
ureolytic cells were immobilized on cotton cloth using polyethyleneimine. the cells when coated with polyethyeneimine were found to flocculate, and the flocs were found to adhere stably to cotton cloth. a contact time of 1 h was found to be necessary for cell binding, even though considerable binding was seen immediately after soaking the cloth in polyethyleneimine-coated cell flocs. the ph optima did not change significantly on immobilization. the cell-bound cloth retained 80 of the original activity, even after 10 reuses for the hydrolysis of urea. these adhered cells have been used in a continuous bioreactor with half-life of 15 days for the degradation of urea. (author abstract) 27 refs
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