섬유

determination of cyclopropenoic fatty acids by reversed-phase liquid chromatography and gas chromatography

  • 출판일1999.03
  • 저자
  • 서지사항
  • 등록일 2016.11.02
  • 조회수 227
a description is given of analyses in parallel by gas-liquid chromatography and high-performance liquid chromatography (glc-hplc) for quantitative analysis of cyclopropenoic fatty acid (cpefa) methyl esters in oils. the separation of the two cpefa (malvalic and sterculic) methyl esters from methyl oleate was carried out by hplc using a reversed-phase column (rp-8). the mobile phase consisted in an acetonitrile-water (85:15, v/v) mixture and quantitation was obtained with an uv detector at 206 nm. the malvalate and sterculate vs. oleate ratio areas where used with oleate results obtained by glc using packed or glass capillary columns for the calculation of fatty acid composition except for the cyclopropenoids. samples of cottonseed and kapok and baobab seed oils were examined. this parallel glc-hplc method was compared to direct glc and hydrobromic titration of cpefa. 17 refs
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