섬유

application of tlc chemical confirmatory tests to minicolumn chromatography of aflatoxins

  • 출판일1999.03
  • 저자
  • 서지사항
  • 등록일 2016.11.02
  • 조회수 245
the minicolumn (mc) proposed by c. e. holaday and j. lansden was developed with a standard aflatoxin solution and with the extracts of corn, rice, wheat, cottonseed, peanut cake and black pepper, each having a different level of aflatoxins. one-half ml each of 2,4-dinitrophenylhydrazine, p-anisaldehyde, 20 h//2so//4, 20 hcl and trifluoroacetic acid (tfa) with 25 hno//3, which were used for confirming aflatoxins on tlc, were applied to the developed column. among these, all the 3 acid reagents changed the blue fluorescence of aflatoxins to yellow and thus were found to be satisfactory confirmatory tests. the tfa with 25 hno//3 had the lowest detection limit - 5 ppb. 13 refs
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