섬유

determination of glutamine and asparagine by isocratic elution reverse phase liquid chromatography with fluorescent detection

  • 출판일1999.03
  • 저자
  • 서지사항
  • 등록일 2016.11.02
  • 조회수 236
a method was developed specifically for the determination of glutamine and asparagine in the presence or absence of other amino acids. the amino acids were derivatized by o-phthalaldehyde/2-mercaptoethanol and separated by isocratic elution with a mobile phase consisting of acetonitrile and sodium acetate buffer. an application of the method for the analysis of glutamine and asparagine in the enzymatic hydrolysate of cottonseed protein is described. 11 refs
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