섬유

enhanced cellulase production in mutants of thermomonospora curvata

  • 출판일1999.03
  • 저자
  • 서지사항
  • 등록일 2016.11.02
  • 조회수 242
thermomonospora curvata, a thermophilic actinomycete, secretes multiple forms of endo- $beta$ , 1-4-glucanase (eg) when grown on a cellulose-mineral salts liquid medium. the eg activity (measured as carboxymethyl cellulose hydrolysis) was separated by ion exchange chromatography into three distinct components which differed in their kinetic properties. exposure of thm. curvata to ultraviolet light, n-nitrosoguanidine, or ethane methyl sulfonate produced mutants with enhanced eg production. selection of colonies which cleared cellulose agar plants containing 2-deoxyglucose or glycerol yielded mutants having 1. 5 to 2. 6 times the extracellular eg and saccharifying activity (measured by filter-paper and cotton-fiber hydrolysis). 40 refs
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