직/편성물 염색

large-scale production and purification of $kappa$ -carrageenase from pseudomonas carrageenovora for applications in seaweed biotechnology

  • 출판일1999.03
  • 저자
  • 서지사항
  • 등록일 2016.11.02
  • 조회수 281
cultures of pseudomonas carrageenovora were grown successfully in a stirred-tank fermentor system suitable for scaled-up conditions improved production of the enzyme $kappa$ -carrageenase was obtained by starting bacterial growth on a cheap carbon source (lactose) and adding small amounts of carrageenan (0.15) to initiate enzyme production. this extracellular enzyme was found to be stable both during fermentation and the following purification steps. for large-scale purification, ultrafiltration (membrane/hollow fiber) followed directly by ion exchange chromatography on the cation exchanger system s-sepharose ff was found most convenient. (author abstract) 21 refs
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