비의류제품

fundamentals of enzyme substrate determinations by fiber optics spectroscopy

  • 출판일1999.03
  • 저자
  • 서지사항
  • 등록일 2016.11.02
  • 조회수 451
optodes and optical enzyme sensors are usually based on the determination of light reflected from the sensor head on an optical fiber. the fundamentals for the mathematical description of the corresponding processes are given by the kubelka-munk function, while in practice the lambert-beer-bouger's law is often applied the response of a ph-optode with phenol red as indicator can be described by so-called 'f-diagrams' derived from the kubelka-munk function: however, the evaluation of the reflection measurements by the lambert-beer-bouger's law also leads to satisfactory results this can be explained by the existing (pseudo-) linearity between the quantities (f/sub lambda / and a/sub lambda /) of both theories the application of this ph-optode in enzyme sensors for urea and penicillin g results in linear calibration curves while in the case of a fluoresceine based electrode nonlinear calibration curves are obtained
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