Researchers investigated the preparation and characterization of crosslinked porous silk fibroin gel (CPEG) to develop new biomedical materials. They used ethylene glycol diglycidyl ether (EGDE) as a crosslinking agent and prepared the CPFG by freezing at -50 to -5 degrees centigrade and thawing a homogeneous fibroin solution containing EGDE. The average pore size of the CPEG ranged from 100-300 microns by adjusting the concentration of silk fibroin, concentration of EGDE, and the freezing temperature. An increase in the concentration of the silk fibroin and EGDE within a certain range improved the tenacity and elongation ratio of CPEG. Results indicated the EGDE was an effective crosslinking agent for the low temperature preparation of CPFG with excellent properties. 8 refs.