[직/편성물] Kynurenine Located within Keratin Proteins Isolated from Photoyellowed Wool Fabric 출판일 : 2006.04.01 저자 : Scott D. Bringans, Jolon M. Dyer, Jeffrey E. Plowman, Warren G. Bryson 서지사항 : Textile Research Journal, Volume 76, Issue 4, 288 페이지 등록일 : 2011.10.26 I 조회수 : 694 작성자 : admin |
This paper describes the identification of the yellow chromophore-containing residue
kynurenine within photoyellowed wool. Photoyellowed Merino wool fabric was tryptically
digested and the resultant peptides were separated by high-performance liquid
chromatography. Peaks with absorbance at 400 nm were collected and analyzed by tandem
quadrupole time-of-flight mass spec-trometry. Kynurenine was identified as a yellow
chromophore in two peptide sequences characteristic of Type 1 intermediate filament and
high glycine-tyrosine wool proteins. To our knowledge this is the first conclusive evidence
confirming the identity of photoyellowed products and their location within the primary
structure of wool proteins. A number of non-yellow modifications were identified in the
course of this study and taken together with the identification of kynurenine modifications,
these results support the current mechanistic theory of the radical oxidative process
occurring during photoyellowing.
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