To optimize the ginning parameters, a study was performed to investigate the influence of leather roller speed, fiber moisture on ginning rate, lint quality and electric energy consumption during ginning on double roller ginning machine. Seed cottons of different staple lengths were ginned at three levels of roller speed and moisture contents and experiments were performed on a specially designed experimental set up, which recorded roller revolutions per minute (RPM) and energy consumption. Duncan's Multiple Range Test revealed that the highest roller speed of 120 RPM coupled with 7 % moisture content of seed cotton showed the highest ginning rate with maximum saving in electric energy. Further, the highest ginning rate and maximum energy saving were observed for higher staple length of fibers as compared to lower staple length of fibers. Fiber properties of lint obtained from different treatments were measured using High Volume Instrument and Advanced Fiber Instrumentation System and results showed that the important fiber parameters remain unaffected.