A series of formulations with varying enzyme and chelator components is tested for flax fiber yield and properties using a recently developed enzyme retting system on Ariane flax grown as a winter crop in southeastern South Carolina. The levels of Viscozyme L, a commercial pectinase-rich enzyme mixture, and Mayoquest 200, a commercial chelator containing 38% ethylenediaminetetraacetic acid (EDTA) as tetrasodium salt, are varied. Enzyme retted flax straw is hand-carded and passed one time through a Shirley Analyzer for cleaning. The chelator level determines the fine fiber (i.e., Shirley cleaned) yield. Fiber strength measured by Stelometer is inversely proportional to enzyme level and not affected by chelator level. Fiber fineness measured by air flow methods is better with higher enzyme levels, and within enzyme levels the higher chelator levels tend to produce fibers with the highest degree of fineness. Relative cost calculations, taking into account fiber yield with costs of enzyme and chelators, provide a framework for determining retting efficiency and fiber quality. Results indicate that fiber properties can be tailored by enzyme or chelator levels. Further, commercial enzyme mixtures and chelators effectively ret flax and can serve as a basis for large scale retting tests.