In this investigation, the behavior of crossover points of spectral reflectance of metameric pairs in the frequency domain has been studied. Also the general metameric index has been calculated using a power spectrum by Fourier transformation. Correlation between these results and the results of current methods was studied. Results show that the transformation of spectral reflectance differences of metameric pairs in the frequency domain could be considerable. This new approach is regarded as a useful method to investigate the metamerism phenomenon.