[직/편성물 염색] Cometabolic Decolorization of the Reactive Azo Dye Orange II by Geobacillus stearothermophilus UCP 986 출판일 : 2009.09.01 저자 : Norma S. Evangelista-Barreto, Clarissa D. Albuquerque, Regine Helena S. F. Vieira, Galba M. Campos-Takaki 서지사항 : Textile Research Journal, Volume 79, Issue 14, 1266 페이지 등록일 : 2011.05.23 I 조회수 : 90 작성자 : admin |
The decolorization of solutions containing the textile reactive dye Orange II by the bacteria
Geobacillus stearothermophilus (UCP 986) was investigated. The experiments were
statistically evaluated by two-level-four factor (2 4) full factorial design, as aeration, azo
dye concentration, inoculum size, and culture media. The best results were with Luria
Bertani (LB) medium under aeration, and the decolorization efficiency meant that 96—98% of
the azo dye was removed after 24 h of incubation. The efficiency of LB medium in color
reduction was due to a co-metabolic process. Sulphanilic acid (4-aminobenzene sulphonic
acid) was not detected. The testing for toxicity using the brine shrimp Artemia salina
showed the absence of toxic metabolites during the decolorization process; however, when
the concentration of the dye and agitation were high (0.050 mM and 150 rpm) the shrimp
showed high mortality rates (LC50 (lethal dose — assay concentration able to cause 50% of
death) 49.28% v/v). The parameters such as culture medium and agitation are significantly
important to the decolorization of the Orange II process. G. stearothermophilus (UCP 986)
showed a high biotechnology potential for textile bioremediation of azo dye under the co-
metabolic process.
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